LCSM is used for the detection and quantitative determination of wild yeast populations in brewing culture yeast. It is designed to encourage the growth of non-Saccharomyces yeast. An inoculum of approximately 1 million culture yeast cells needs to be plated on LCSM. While the growth of culture yeast will be suppressed by cupric sulfate, wild yeast will develop as large, distinct colonies. A few Saccharomyces, like S. cerevisiae var. diastaticus, may be more resistant to cupric sulfate and show a certain level of growth.
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